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101.
Order of herbivore arrival on wild cabbage populations influences subsequent arthropod community development 下载免费PDF全文
In plant–arthropod associations, the first herbivores to colonise a plant may directly or indirectly affect community assembly on that particular plant. Whether the order of arrival of different arthropod species further modulates community assembly and affects plant fitness remains unclear. Using wild Brassica oleracea plants in the field, we manipulated the order of arrival of early‐season herbivores that belong to different feeding guilds, namely the aphid Brevicoryne brassicae and caterpillars of Plutella xylostella. We investigated the effect of herbivore identity and order of arrival on community assembly on two B. oleracea plant populations during two growth seasons. For this perennial plant, we evaluated whether foliar herbivory also affected herbivore communities on the flowers and if these interactions affected plant seed production. Aphid infestation caused an increase in parasitoid abundance, but caterpillars modulated these effects, depending on the order of herbivore infestation and plant population. In the second growth season, when plants flowered, the order of infestation of leaves with aphids and caterpillars more strongly affected abundance of herbivores feeding on the flowers than those feeding on leaves. Infestation with caterpillars followed by aphids caused an increase in flower‐feeding herbivores compared to the reversed order of infestation in one plant population, whereas the opposite effects were observed for the other plant population. The impact on plant seed set in the first reproductive year was limited. Our work shows that the identity and arrival order of early season herbivores may have long‐term consequences for community composition on individual plants and that these patterns may vary among plant populations. We discuss how these community processes may affect plant fitness and speculate on the implications for evolution of plant defences. 相似文献
102.
103.
Spinach plants ( Spinacia oleracea L. cv. Subito) were grown in a complete nutrient solution under ample light intensity (14 h day−1 at 660 μmol m−2 s−1 ) before being transferred either to a minus-N solution (experiment 1), or to limiting light conditions (6 h day−1 at 220 μmol m−2 s−1 ; experiment 2). Shoot growth in experiment 1 decreased significantly from 0.24 day−1 to 0.07 day−1 after the fourth day of transfer. Root relative growth rate increased after 1 day from 0.25 to 0.31 day−1 , but decreased on the fifth day after transfer to 0.11 day−1 . Shoot growth in experiment 2 decreased significantly from 0.25 to 0.17 day−1 after the fourth day of transfer, while root growth decreased to half of its original level (0.25 day−1 ) already on the second day. Growth substrate levels in the plants (free sugars, free amino acids) and starch levels depended on the plant age, the moment in the diurnal cycle, and the imposed treatment. Fluctuations in shoot growth or root growth resulting from the light or N limitation could not be explained by a correspondent increase or decrease in the levels of growth substrates. The hypotheses underlying the functional equilibrium theory, assuming shoot and root growth to be controlled by N- and C-containing substrates respectively, and several other growth and partitioning models are therefore questioned. A neglect of the osmotic role of the free sugars in these models might be the explanation for this. 相似文献
104.
The possibilities of independent function of the two chaperonin 10 (cpn10) domains of the cpn10 homologue from spinach chloroplasts and the role of five conserved amino acid residues in the N-terminal cpn10 unit were investigated. Recombinant single domain proteins and complete chloroplast cpn10 proteins carrying amino acid exchanges of conserved residues in their N-terminal cpn10 domain were expressed in Escherichia coli and partially purified. The function of the recombinant proteins was tested using GroEL as chaperonin 60 (cpn60) partner for in vitro refolding of denatured ribulose-1,5-bisphosphate carboxylase (Rubisco). Interaction with cpn60 was also monitored by the ability to inhibit GroEL ATPase activity. In vitro both isolated cpn10 domains were found to be incapable of co-chaperonin function. All mutants were also severely impaired in cpn10 function. The results are interpreted in terms of an essential role of the exchanged amino acid residues for the interaction between co-chaperonin and cpn60 partner and in terms of a functional coupling of both cpn10 domains.To test the function of mutant chloroplast cpn10 proteins in vivo the cpn10 deficiency of E. coli strain CG712 resulting in an inability to assemble -phage was exploited in a complementation assay. Transformation with plasmids directing the expression of mutant chloroplast cpn10 proteins in two cases restored -phage assembly in this bacterial strain to the same extent as did transformation with a plasmid encoding wild-type cpn10 protein. In contrast a plasmid encoded third mutant and truncated forms of chloroplast cpn 10 showed significantly reduced complementation efficiencies. 相似文献
105.
T. Cardi E. D. Earle 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(2):204-212
New types of cytoplasmic male sterility (CMS) in Brassica oleracea would be useful for F1 hybrid seed production. The `Anand' cytoplasm derives from the wild species B. tournefortii. Rapid cycling stocks of B. rapa and B. oleracea were used in cybridization experiments as donor and recipient of `Anand' (=`tour') CMS, respectively. Prior to fusion with
PEG, donor protoplasts were inactivated with 30 krad γ-rays and recipient ones with 3 mM iodoacetate, respectively. No calli were obtained from the pre-treated protoplasts. The frequency of shoot regeneration was
21–43% in untreated B. oleracea controls, but only 0–0.5% in `Anand' B. rapa. Putative cybrids were regenerated from about 3% of the calli from fused protoplasts. Regenerated plants were analyzed for
nuclear DNA content, plant and flower morphology, pollen production, female fertility, cold tolerance, and organelle composition.
Eighty-one percent of the regenerated controls and 63% of fusion-derived plants were diploid. The rest showed DNA contents
corresponding to 2x–4x, 4x, or higher ploidy levels, presumably due to somatic doubling in vitro and/or fusions in which the donor nucleus was not completely
eliminated. Sixty-four percent of the cybrids had stamens and petals varying in size and shape and were male-sterile, with
indehiscent anthers. Their phenotype was otherwise similar to that of B. oleracea. The remaining plants had normal flowers and were male-fertile. Data from crosses with fertile pollinators indicated good
female fertility in some of the sterile lines, both after hand and insect pollinations in cages. Mitochondrial (mt) segregation
in the cybrids was slightly biased towards `Anand' mitochondria, and the presence of `Anand' mtDNA fragments was strongly
associated with male sterility. Evidence of mtDNA rearrangements was obtained in some cybrids. Segregation of chloroplasts
was slightly biased towards B. oleracea. The presence of `Anand' chloroplasts with a B. oleracea nucleus did not result in cold temperature chlorosis, as seen in `Ogura' CMS plants.
Received: 22 February 1996 / Accepted: 10 May 1996 相似文献
106.
A.A. El-Khatib 《Biologia Plantarum》1997,40(3):425-431
Indoor experiments demonstrated that allelopathic potential of rosette and flowering plants of qort is an important factor
explaining the growth reduction of its associated species. Aqueous tissue extracts of flowering plants exhibited strong inhibitory
effects on the germination percentage and radicle growth rate of the tested species as compared with those of vegetative plants.
Under laboratory conditions, this inhibition was in agreement with toxicity assessments of soil samples collected from the
rhizosphere of T. resupinatum L., where shoot and root dry mass of the tested species were significantly reduced. Detoxification
of allelochemicals by presence of activated carbon can eliminate the inhibitory effects of the different extracts. This technique
clarifies the occurrence of allelopathic interference by qort on seed germination and seedling growth, and hence suspects
the allelopathic potential of qort in the growth reduction of associate species under field conditions along with competition.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
107.
R. A. Cole 《Entomologia Experimentalis et Applicata》1997,85(2):121-133
This paper describes an experiment to investigate the relative importance of glucosinolates and the availability of free amino acids in the phloem to the feeding behaviour and development of the specialist brassica aphid, Brevicoryne brassicae (L) (cabbage aphid) and the generalist, Myzus persicae, (Sultzer) (peach potato aphid). Aphid development was determined on brassica species and cultivated brassica varieties. Analysis of individual glucosinolates in the wild brassica species identified significant differences in their profiles and in their concentrations present in freeze dried leaves. Multiple regression analysis showed a significant correlation (
) between the intrinsic rate of increase of B. brassicae and glucosinolate concentrations; four glucosinolates, 2-OH-3-butenyl, and 2-propenylglucosinolate, 3-methoxyindolyl and 4-pentenylglucosinolates accounted for 79% of the variation. The intrinsic rate of increase of M. persicae was less correlated, though still significant (
); 3-indolyglucosinolate and 3-butenylglucosinolate accounted for 47% of the variation. Regression analysis also showed a correlation between phloem amino acid concentrations and the intrinsic rate of increase of B. brassicae (
) but not of M. persicae. The concentration of the important amino acids tyrosine, alanine, leucine and glutamic acid, accounted for 43% of the variation in intrinsic rate of increase. Leaf water potential of the Brassica species showed no relationship with the intrinsic rate of increase of either B. brassicae or M. persicae. 相似文献
108.
109.
H. A. Bell R. E. Down A. E. Kirkbride-Smith J. P. Edwards 《Journal of Applied Entomology》2004,128(8):548-553
Abstract: The predatory behaviour of Podisus maculiventris was investigated when this bug was presented with Lacanobia oleracea larvae infected with the microsporidian pathogen Vairimorpha necatrix . In choice tests, adult predatory bugs attacked V. necatrix -infected L. oleracea prey in similar numbers to uninfected larvae. Exposure to infected prey during nymphal development increased the rate at which adult bugs attacked diseased L. oleracea larvae. Fifth instar P. maculiventris nymphs, however, attacked infected prey in the majority of cases (>80% of occasions). Consumption of healthy and infected prey was measured for both adult and nymphal bugs. Over the course of 1 week, the mean number of V. necatrix -infected prey eaten by P. maculiventris adults (7.0 ± 0.82) was approximately twice the number of uninfected prey consumed (3.8 ± 0.42). Similarly, the number of prey larvae attacked by the bug over the course of the final nymphal stadium was also increased, with 2.9 ± 0.42 uninfected larvae eaten as opposed to 4.9 ± 0.27 V. necatrix -infected prey. However, small-scale investigations into the rate of P. maculiventris reduced small populations of L. oleracea indicated that the combination of the predator and pathogen would produce, at best, an additive effect. 相似文献
110.
Fluxes of atmospheric hydrogen sulphide to plant shoots 总被引:4,自引:2,他引:2